GESAN AST-GOT LR

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Kit for measurement of aspartate-aminotransferase in serum or plasma – Kinetic UV optimized method IFCC* * International Federation of Clinical Chemistry and Laboratory Medicine

Summary

AST measurements are used in the diagnosis and treatment of certain types of liver and heart disease.

Principle

The enzyme aspartate-aminotransferase (AST) (or glutamic-oxaloacetic transaminase/GOT) catalyzes reaction between alpha-ketoglutarate and L-aspartate giving glutamate and oxaloacetate. In presence of malate dehydrogenase (MDH), oxaloacetate reacts with NADH giving malate and NAD+. The rate of decrease of absorbance due to oxidation of NADH to NAD+ is directly proportional to sample AST activity.

The enzyme aspartateaminotransferase (AST) (or glutamicoxaloacetic transaminase/GOT) catalyzes reaction between alphaketoglutarate and Laspartate giving glutamate and oxaloacetate. in presence of malate dehydrogenase (MDH), oxaloacetate reacts with NADH giving malate and NAD+. The rate of decrease of absorbance due to oxidation of NADH to NAD+ is directly proportional to sample AST activity.

Reagents

  1. R1
    • Goods buffer pH 7.8 80.0 mmol/l
    • L-aspartate 240.0 mmol/l
    • LDH 1800 U/l, MDH ≥ 800 U/l
  2. R2
    • Goods buffer pH 7.8 80.0 mmol/l
    • alpha-ketoglutarate 65.0 mmol/l
    • NADH ≥ 1.18 mmol/l

Kemenkes RI AKL 20101719340

Graha Megatama Indonesia
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