Use
Kit for measurement of direct bilirubin in serum or plasma. Colorimetric method Jendrassik – Grof modified.
Summary
Direct bilirubin measurements are used in the diagnosis and treatments of various liver diseases, and metabolic disorders.
Principle
End point analysis. Direct (conjugated) bilirubin reacts with sulphanilic acid and giving a coloured azocompound (azobilirubin). The increase in absorbance due to the formation of azobilirubin is proportional to the direct bilirubin concentration in the sample.
Reagents
- R1
- Sulphanilic acid 22.0 mmol/l preservatives and surface-active agents not anionic
- R2
- Nitrite sodium 0.35 mmol/l
Kemenkes RI AKL 20101915797
English 
Indonesian