Kit for measurement of total bilirubin in serum or plasma. Colorimetric method Jendrassik – Grof modified.
Total bilirubin measurements are used in the diagnosis and treatments of various liver diseases, and haemolytic and haematology disorders.
End point analysis. Total bilirubin reacts with sulphanilic acid, giving a coloured azocompound (azobilirubin). The increase in absorbance due to the formation of azobilirubin is proportional to the total bilirubin concentration in the sample.
- Sulphanilic acid 12.5 mmol/l
- citric acid 1.00 mol/l
- caffeine 0.18 mol/l
- Preservatives and surface-active agents not anionic
- Nitrite sodium 0.52 mmol/l