Use
Kit for measurement of alanine-aminotransferase in serum or plasma. Kinetic UV optimized method IFCC* * International Federation of Clinical Chemistry and Laboratory Medicine
Summary
ALT measurements are used in the diagnosis and treatment of certain types of liver and heart disease.
Principle
The enzyme alanine-aminotransferase (ALT) (or glutamic-pyruvic transaminase/GPT) catalyzes reaction between alpha-ketoglutarate and L-alanine giving glutamate and pyruvic acid. In presence of lactate dehydrogenase (LDH), pyruvic acid reacts with NADH giving lactic acid and NAD+. The rate of decrease in absorbance due to the oxidation of NADH to NAD+ is directly proportional to sample Alt activity. In the reagent is contained also LDH to convert the endogenous pyruvate into lactate during the preincubation.
Reagents
- R1
- Good buffer pH 7.5 80.0 mmol/l
- L-alanine 500.0 mmol/l
- LDH ≥ 1500U/l
- R2
- Good buffer pH 7.5 80.0 mmol/l
- alpha-ketoglutarate 65.0 mmol/l
- NADH ≥ 1.18 mmol/l
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