VITRO HPV Direct Flow Chip Kit

Use

HPV Direct Flow CHIP is an in vitro diagnostic kit for the human papillomavirus (HPV). The infection with HPV is an essential factor in cervical and anogenital carcinogenesis.

Principle

The HPV Direct Flow CHIP kit methodology is based on the amplification of a fragment in the viral region L1 of papillomavirus by PCR, followed by hybridization onto a membrane with DNA specific probes by using the DNA-Flow technology both for automatic and manual hybriSpot platforms. The biotinylated amplicons generated after the PCR are hybridized in membranes containing an array of specific probes for each target as well as amplification and hybridization control probes. The DNA-Flow technology allows a very fast binding of the PCR product and its specific probe in a three-dimensional porous environment, as compared to the hybridization in a conventional surface. Once the binding between the specific amplicons and their corresponding probes has occurred, the signal is visualized by an immunoenzymatic colorimetric reaction with Streptavidin−Phosphatase and a chromogen (NBT-BCIP) generating insoluble precipitates in the membrane in those positions in which there has been hybridization. The results are analyzed automatically with the HybriSoft™ software.

Storage And Stability Conditions

HPV Direct Flow Chip consists of two components that are supplied in separate boxes:

PCR reagents: Shipment at 2-8 °C. Upon receipt, they must be stored at -8 °C. They will be stable until the specified expiration date. The PCR reagents must be stored in areas free of DNA or PCR products contamination. Once the package containing the tubes strip with the lyophilized PCR mix is opened, store the remaining tubes up to a maximum of one week at 2-8°C in the original package. Hybridization reagents. Shipment at 2-8 °C. Upon receipt, they must be stored at -8 °C. They will be stable until the specified expiration date. The reagents as well as the HPV Chips are stable until the specified expiration date. Do not freeze. The hybridization reagent A must be pre-heated in a thermostatic bath or heater (only before using it in the manual equipment HS12) at 41°C before its use While the rest of the hybridization reagents must be used at room temperature (20-25°C).

Please refer to the user manual above for additional information

Kemenkes RI AKL 20306026437

HPV Direct Flow Chip Kit (PCR Reagents) KEMENKES RI AKL 20306026437

HPV Direct Flow Chip Kit (Hybridization Reagents)  KEMENKES RI AKL 20306026545