Use
Kit for measurement of uric acid in serum, plasma and urine
Colorimetric enzymatic method Uricase-POD-PAP.
Summary
Uric acid measurements are used in the diagnosis and treatment of gout and impaired renal fuction.
Principle
End point analysis. Uric acid is converted by uricase and H2O2 which, under the catalytic influence of peroxide (POD), oxidizes compound, reacts with 4 aminophenazone and 3,5-diclorophenol-sulphonate giving a red coloured compound. The increase in absorbance generated by the red dye is proportional to the uric acid concentration in the sample.
Reagents
- R1
- Goods buffer pH 8.0 100.0 mmol/l
- ascorbate oxidase ≥ 200 U/l
- 3,5-diclorophenol-sulphonate 2.5 mmol/l
- R2
- Goods buffer pH 8.0 100.0 mmol/l
- 4-aminophenazone 0.8 mmol/l
- Peroxidise ≥ 3000 U/l
- Uricase ≥ 600 U/l
Kemenkes RI AKL 20101127362
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