Kit for measurement of tryglicerides in serum or plasma. Colorimetric enzymatic method GPO – PAP.
Tryglicerides measurements are used in the diagnosis and treatment of hyper – lipidemia.
Tryglicerides are hidrolized, in presence of lypoproteinlipase (LPL), into fat acid and glycerol which is transformed, by glycerolchinase (GK), ATP and glicerol-3-P-oxidase (GPO), into diidroxiaceton-phosphate and H2O2. The hydrogen peroxid catalyzed from peroxidase (POD) reacts with 4-aminophenazone and 4-phenol-chloride giving a coloured compound whose intensity is proportional to the concentration of triglycerides in the sample
- PIPES buffer 100.0 mmol/l
- phenol 16.0 mmol/l
- lipoproteinlipase ≥ 4000 U/l
- glicerolkinase ≥ 2000U/l
- peroxidase ≥ 2500U/l
- ATP 0.8 mmol/l
- 4-aminophenazone 1.4 mmol/l
- glicerol-3-P-oxidase ≥ 2000 U/l
KEMENKES RI AKL 10101715341